A quick diversion into fashion.

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Busy busy tracing biological forms.  Just received a shipment of another 30 lenses. zoologische

Finished installing the fresnel sculptures and they look great.  There is this odd sense of frozen time, where neither the projected image or the sculptures are moving, but the light is bending and moving through the lenses according to your movement.    IMG_8996.jpg

Things look really trippy from the other side of that lens.  I may put a large lens in front of the laser cut lenses:IMG_9313.jpgIMG_9308.jpgIMG_9367.jpgIMG_9339.jpg

Below: the first batch of fresnels.  Took some time to get the settings right since what I thought were the old settings were scorching my new brand of lenses- eventually got it by dialing down the power and putting blue painters tape on the back, transfer tape on the front to protect.  Imagery from invertebrate biology textbooks and the biodiversity heritage library.IMG_8959.JPGimg_8943Video:

img_8925IMG_8973.JPGA scorched one:img_8903My tests:img_8905:

I’ll be trained on the laser cutter next week!  While I’m focusing on cutting fresnels and transparent materials, I couldn’t help but gawk at some of these laser cut paper sculptures.  The one right below looks like pretty mold!  While I don’t aspire to the level of these artworks, It’s really quite amazing what is possible now.

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Some inspiration for my own cuts: Haeckel_Spumellaria.jpg64d46a8d0ff41ab0ebdd53ec352655cc.jpg9992f3c29f8c81c140f515bd2c68bfe0.jpgi-27d4775dda188d3c4c57ce61e8cb67c8-Arctic Creature 6 - nemertean pelagonemertes rollestoni.jpg7501c406670ce2f9f2ef87d3f0398dad.jpgearthwormcsl-14B8A4ABC7C2C986625.jpgstock-photo-a-low-power-view-of-the-entire-cross-section-of-the-small-intestine-of-a-guinea-pig-showing-villi-43653424.jpg10.pngchelicerata nervous system.jpgDemospongiae_spicule_diversity.png

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I love transparent and optically interesting materials- I’ve been experimenting with printing clear objects which could be used to add sculptural depth to my laser cut fresnel-sculptures.  Colorfabb HT clear filament rocks!  It is quite strong and heat resistant (have to extrude at 260C), and has a high flow rate so seeps into air pockets which make other clear filaments turn cloudy.  With little to no fine tuning I produced the prints below, 100% infill of course.  Not perfectly clear, but pretty darn good.  I thought I’d have to have access to an SLA machine to do anything like this.  See: http://learn.colorfabb.com/lets-make-something-clear/

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IMG_8794.jpgReally love this clear filament- it prints pretty great also, atleast for these smaller objects- no warping or edge curling even though I’m not using an enclosure or cooling.  It is sticking well to PET tape @120C supplemented with wolfbite adhesive from matterhackers. At 0.1mm layer height and 15mm/s speed these are very slow prints though!

Got it printing pretty well over winter break!  In the beginning had trouble with layer shifts until I figured out a set screw was loose on my left motor- after that it was relatively minor fixes for stability.  I’ve been printing mostly ABS since me and PLA do not get along- below are my ABS (left) and PLA (right) benchys.  It is apparently possible to print ABS w/out enclosure if you use a ton of glue, also have been experimenting with the ‘enable draft shield’ option in Cura.  benchy.png

More prints (STLs available here: http://www.thingiverse.com/pinkbeetle/makes)dc5514c23e8427ce95b406c4f4d7c7e3_preview_featured.JPG

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^Quite a bit better then my first prints!:IMG_8062.JPG

3dprinter_jessholz-4Got the new extruder- extruding much better now, there was definitely something loose in the old one.  I can get it to print a few layers of ABS but it then the print comes loose off the bed, so still needs tweaking.  My x-y is a little tight compared to Cameron’s and it’s making the motor drivers overheat (thankfully the smoothieboard protects itself by turning off)- hopefully can adjust the wheel tension ok.

EDIT: xy fixed!  Lionel noticed that one of the bolts from the belt clamps was protruding a little far and scraping the v-slot underneith.  Thank you Lionel for noticing that as I was about to take my carriage apart to put new spacers in.  Can’t wait to get this thing printing!  Video below is from before the xy was fixed, it was pointed out online that it might be skipping steps:

I spent a long time on brackets for the power supplies and electronics housing which attached to the outside of the frame, so I could eventually add an enclosure:

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Other then the smoothieboard the main upgrades were the 575w heated bed and 3 point leveling.  Hopefully can get her printing over winter break.

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So eventually I got my d-bot moving:

After a few erroneous steps per mm calculations it was still underextruding pretty bad so I checked the hotend, sure enough the heat block wasn’t tight.  I went to tighten it and accidentally ripped the thermistor off it’s wires.  So my printer is dead until next week when I get another hot end ($$$).

A sad looking print, the only one it’s made thus far:

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I love the blebby membrane on this cell.  Blebs (the bubbly structures) can be a result of apoptosis, otherwise known as programmed cell death, where the membrane blebs start budding off into space, filled with cell junk.  However in this case this case I think this type of cultured cells (CHO cells: Chinese Hamster Ovary)  just sort of have a bubbly membranes a lot; they are not apoptotic.

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The 3d reconstruction is made based on stereo pairs of images: two images at 5-10 degrees difference in tilt that could be viewed through a stereoscope, or computed into a 3d model.  Typically the reconstruction is done from two images near the top, with this cell the reconstruction was from two images on one side.  You can see the resulting distortion below.  But still looks like the cell when viewing from the angle it was reconstructed from.

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